等溫滴定量熱儀（Isothermal Titration Calorimetry）

型號👨‍🏭：MicroCal? iTC200

中文介紹🧑🏼‍🎄：

ITC是一種通用屬性技術（測量熱量），廣泛應用於二次篩選/hit 驗證中快速得到 KD 值，指導優化和表征分子間作用機理🏌🏼‍♂️。 它還可以用於驗證常用參數IC50和EC50的值。 IC50是最大抑製濃度的一半，是化合物在抑製生物學或生化功能中的有效量度。 EC50，是最大有效濃度的一半，通常用作藥物效力的量度。 此外🖖🏻，ITC可用於測量和量化涉及熱變化的任何過程，例如酶促反應。

英文介紹：

ITC is a universal property technique (measure heat) and thus is used across a wide range of applications from measuring affinity values for secondary screening confirmation of hits and hit validation to guiding lead optimization and characterizing mechanism of action. It´s also used to validate the values of the commonly used parameters IC50 and EC50. Briefly you can say that IC50 is the half maximal inhibitory concentration and is a measure of the effectiveness of a compound in inhibiting biological or biochemical function. It is comparable to EC50 which is the half maximal effective concentration, commonly used as a measure of drug´s potency. Furthermore, ITC can be used to measure and quantify any process that involves a heat change, such as an enzymatic reaction.